Proteomics: is the large-scale study of proteins in biological systems. The proteome is the entirety of protein components, including the modifications made to a particular set of proteins, produced by an organism or system. This proteome may vary with time and suffer modifications under different experimental conditions or stress, that a cell or organism undergoes.

Infrastructure in the facility is centred on state-of-the-art mass spectrometry for MS and LC-MS/MS experiments and includes chromatographic and electrophoretic systems for protein and peptide separation.


The service integrates different technologies:

  • Gel-based Proteomics
  • MS-based Proteomics
  • Interactomics

Proteomics provide the ability to implement optimized methods to profile proteins in biological fluids and tissues, to quantify proteins, to identify protein, posttranslational modifications, and to study protein-protein interactions and we also have the capability to modify and develop entirely new assays as required by our clients.


Protein identification
Shotgun proteomics (identification of proteins in complex mixtures)
Targeted proteomic profiling (SRM assays)
Relative and absolute quantitation
Post-translational modification analysis
2-D PAGE (Two-dimensional polyacrylamide gel electrophoresis) and 2-D DIGE
Characterization of affinity purified or immunoprecipitated proteins and complexes
Characterization of intact recombinant proteins
MALDI tissue imaging
Bacterial Identification
Molecule-molecule interaction
Luminex bead array


  • 2 Agilent 6490 with iFunnel technology Triple quandrupole LC/MS
  • 1 Agilent 6550 iFunnel Q-TOF LC/MS
  • 1 Thermo Orbitrap Velos Pro
  • Agilent 2100 Bioanalyzer
  • NanoDrop ND-2000 spectrophotometer
  • EXQuest spot cutter: fast, hands-free gel excision
  • Bioplex 200 bead array instrument
  • Biacore 3000 analyzer: Protein-molecule interaction service.
  • Bravo liquid handling robot
  • Imaging systems:
    • Pharos XP: Imager and Scanner for fluorescence, chemiluminescence, chemifluorescence, densitometry, and gel documentation
    • ChemiDoc: imager CCD instrument for chemiluminescence


Proteomic Sotwares

  • Mascot (MatrixScience)
  • SpectrumMill (Agilent)


  • SameSpots (Progenesis)
  • PDQuest Advanced (Biorad)
  • ImageMaster 2D Platinum (GE)

Imaging Systems

  • Quantity One (Biorad)
  • ImageLab (Biorad)
  • ImageQuant TL (GE)

Bioplex 200 protein bead array

  • BioPlex manager (Biorad)

UltrafleXtreme MALDI-TOF/TOF

  • ProteinScape (Bruker)
  • FlexImaging (Bruker)
  • Biotools (Bruker)
  • ClinProTools (Bruker)
  • WARP-LC (Bruker)
  • MALDI-Biotyper (Bruker)
  • MALDI-TLC (Bruker)

Orbitrap Velos Pro ETD

  • Proteome Discover (Thermo)
  • Mass Frontier (Thermo)
  • SIEVE (Thermo)

Biacore 3000 analyzer

  • Bioevaluation (GE)

General Softwares

  • BioNumerics  (Applied-maths)
  • Gene Spring GX  (Agilent)