Protein identification

  • Molecular weight determination for intact purified proteins
  • Peptide Mass Fingerprinting with a MALDI-TOF
  • Tandem MS/MS, with protein/peptide pre-fractionation prior to MS/MS analysis
  • single dimension (nanoLC-MS/MS)
  • Multidimensional protein/peptide fractionation by Liquid Chromatography (LC/LC-MS/MS)
  • Off-Gel Electrophoresis (OGE) Isoelectric Focusing (IEF) and Liquid Chromatography (OGE and IEF-LC-MS/MS )
  • Gel-based pre-fractionation: 2-D gel electrophoresis or 1D-gel electrophoresis (2D-LC-MS/MS or GeLC-MS/MS)
  • Use of multiple MS fragmentation mechanisms (CID, ETD, etc)

< back
Shotgun proteomics (... >